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Waters, Milford, MA) were equilibrated with 6 mL methanol and 2 mL H 2 O before samples were loaded onto columns

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TB-500 and TB500 naming should be connected to thymosin beta-4 fragment context, with UniProt identifying thymosin beta-4 as the TMSB4X protein and analytical literature discussing N-acetylated LKKTETQ as an active-region marker [11] [14]