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Then incubated with fluorescent secondary antibody (anti-rabbit Alexa 555 or anti-mouse FITC, Beyotime) in the dark at room temperature for 2 h

GLP-1 drugs have been around for more than a decade to help people manage type 2 diabetes, but the ones developed in recent yearsOzempic, Wegovy, Mounjaro, and Zepboundhave exploded in popularity after becoming the worst-kept celebrity weight-loss secret

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The present study emulated a target trial to avoid immortal time and selection bias, chose a second-line glucose-lowering therapy as an active comparator to reduce confounding by indication, used an outcome definition based only on primary care records to minimize detection bias due to differential hospitalizations, and implemented a lag time to mitigate bias due to reverse causality

Retatrutide has GLP-1 activity, but it is not only a GLP-1 drug