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Western blot Equal amounts (50 g) of RINm5F, INS-1, and MIN6 cell lysate were analyzed by Western blotting with rabbit anti-AMPK monoclonal antibody#ab32047 (Epitomics Technology, Berlingame, CA), rabbit anti-phospho-AMPK(Thr172) monoclonal antibody #2531 (Cell Signaling Technology, Beverly, MA), rabbit anti-PARP polyclonal antibody#9542S, anti-cleaved caspase-3, phospho-CREB/CREB, PDX1, phospho Akt/Akt and GAPDH (Cell Signaling Technology, Beverly, MA), respectively

While learning about the reconstitution solution vs bacteriostatic water, keep in mind that as the solution contains 0.9% benzyl alcohol as a bacteriostatic preservative, it does not allow bacteria, viruses, or fungi to develop when the product is stored for 28 days

4 and 5, Extended Data Fig

The fact that adding cagrilintide produced meaningfully greater weight loss over just 32 weeks confirmed the additive effect of the dual mechanism