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The sensor fluorescence response was measured over a time course and an oxygen ramping script mode together with the ACU was used to apply an oxygen gradient by flushing the measurement chamber with nitrogen gas (99.998 % v/v, Westfalen AG, Mnster, Germany)

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2 Materials and methods 2.1 Botanical drug authentication and aqueous extraction The powdered biomass used, Spirulina platensis (Product Number 741094) was bought from Sigma-Aldrich Chemicals Co., St